导师介绍

分子医学硕士生导师吴柏星介绍

个人简介:

2020年中山大学“百人计划“引进人才,中山大学附属孙逸仙纪念医院基础与转化医学研究中心副研究员。博士毕业于复旦大学生物化学与分子生物学专业,随后在南方科技大学从事博士后研究工作,合作导师为美国科学院院士Dinshaw J. Patel。

科研工作:

  主要从事核酸相关蛋白的结构生物学研究。主要研究方向包括:(1)RNA与DNA修饰相关蛋白; (2)非编码RNA生成相关蛋白; (3) DNA复制与损伤修复相关蛋白等核酸-蛋白复合物的结构生物学研究。主要研究内容:利用X-射线晶体学以及Cryo-EM技术,结合生物化学与分子生物学方法,研究核酸修饰相关识别因子的结构,如m6A,m5C,ac4C等修饰的的识别与催化机制;与重要疾病相关的RNA结合蛋白(如渐冻人症)以及与非编码RNA生成相关的RNA结合蛋白的结构与功能,另外课题组也对DNA复制与损伤修复相关蛋白复合物的进行研究,旨在阐明真核生物DNA复制相关蛋白质机器的工作机理。截至2020年共发表论文18篇,研究工作主要发表在Nature Communications,Nucleic Acids Research,Molecular Cell,Proc Natl Acad Sci U S A ,Journal of Virology等。 

著作、论文、成果:

主持科研项目:

[1] 中山大学“百人计划”启动项目;资助经费:50(万元);批准年度:2020年;项目起止年月:2020-06至2023-06(在研)

[2] 国家自然科学基金青年科学基金项目;资助经费:15(万元);批准年度:2019年;项目起止年月:2020-01至2021-12(在研)

代表性论文:

1) Hou Y.#, Wu B.#, Sun J.#, Gao Y.#; Nie H.; Nie Z.; Quan S.; Wang Y.*; Cao X.*, Li S.*; Arabidopsis CPSF30-L regulates nitrate signaling by controlling alternative polyadenylation through binding to m6A. (In revision)

2) Wang N#, Bao H#, Chen L, Liu Y, Li Y, Wu B*, Huang H*. Molecular basis of abasic site sensing in single-stranded DNA by the SRAP domain of E. coli yedK. Nucleic Acids Res. 2019 Nov 4;47(19):10388-10399. (IF 11.5)

3) Yang Y#, Wang L#, Han X#, Yang WL#, Zhang M#, Ma HL, Sun BF, Li A, Xia J, Chen J, Heng J, Wu B, Chen YS, Xu JW, Yang X, Yao H, Sun J, Lyu C, Wang HL, Huang Y, Sun YP, Zhao YL, Meng A, Ma J*, Liu F*, Yang YG*. RNA 5-Methylcytosine Facilitates the Maternal-to-Zygotic Transition by Preventing Maternal mRNA Decay. Mol Cell. 2019 Sep 19;75(6):1188-1202.e11. (IF 15.58)

4) Wu B#*, Zhang D#, Nie H#, Shen S, Li Y, Li S*. Structure of Arabidopsis thaliana N6-methyl-AMP deaminase ADAL with bound GMP and IMP and implications for N6-methyl-AMP recognition and processing. RNA Biol. 2019 Oct;16(10):1504-1512. (IF 5.35)

5) Qiu Q#, Mei H#, Deng X#, He K#, Wu B#, Yao Q, Zhang J, Lu F, Ma J*, Cao X*. DNA methylation repels targeting of Arabidopsis REF6. Nat Commun. 2019 May 2;10(1):2063. (IF 12.12)

6) He J#, Ye W#, Choi DS#, Wu B, Zhai Y, Guo B, Duan S, Wang Y, Gan J, Ma W*, Ma J*. Structural analysis of Phytophthora suppressor of RNA silencing 2 (PSR2) reveals a conserved modular fold contributing to virulence. Proc Natl Acad Sci U S A. 2019 Apr 16;116(16):8054-8059. (IF 9.41)

7) Yao Q#, Cao G#, Li M, Wu B, Zhang X, Zhang T, Guo J, Yin H, Shi L, Chen J, Yu X, Zheng L, Ma J*, Su YQ*. Ribonuclease activity of MARF1 controls oocyte RNA homeostasis and genome integrity in mice. Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):11250-11255. (IF 9.41)

8) Li Y#, Wu B#, Liu H, Gao Y, Yang C, Chen X, Zhang J, Chen Y, Gu Y, Li J, Ma J, Gan J*. Structural basis for multiple gene regulation by human DUX4. Biochem Biophys Res Commun. 2018 Nov 10;505(4):1161-1167. (IF 2.98)

9) Liu H, Wang R, Yu X, Shen F, Lan W, Haruehanroengra P, Yao Q, Zhang J, Chen Y, Li S, Wu B, Zheng L, Ma J, Lin J, Cao C, Li J*, Sheng J*, Gan J*. High- resolution DNA quadruplex structure containing all the A-, G-, C-, T-tetrads. Nucleic Acids Res. 2018 Nov 30;46(21):11627-11638. (IF 11.5)

10) Wu B#*, Zhang M#, Su S, Liu H, Gan J, Ma J*. Structural insight into the role of VAL1 B3 domain for targeting to FLC locus in Arabidopsis thaliana. Biochem Biophys Res Commun. 2018 Jun 22;501(2):415-422. (IF 2.98)

11) Li W#, Wu B#, Soca WA, An L. Crystal Structure of Classical Swine Fever Virus NS5B Reveals a Novel N-Terminal Domain. J Virol. 2018 Jun 29;92(14):e00324-18. (IF 4.50)

12) Wu B#, Su S#, Patil DP, Liu H, Gan J, Jaffrey SR, Ma J. Molecular basis for the specific and multivariant recognitions of RNA substrates by human hnRNP A2/B1. Nat Commun. 2018 Jan 29;9(1):420. (IF 12.12)

13) Liu H#, Yu X#, Chen Y, Zhang J, Wu B, Zheng L, Haruehanroengra P, Wang R, Li S, Lin J, Li J, Sheng J, Huang Z*, Ma J*, Gan J*. Crystal structure of an RNA- cleaving DNAzyme. Nat Commun. 2017 Dec 8;8(1):2006. (IF 12.12)

14) Wu B#*, Xu J, Su S, Liu H, Gan J, Ma J*. Structural insights into the specific recognition of DSR by the YTH domain containing protein Mmi1. Biochem Biophys Res Commun. 2017 Sep 16;491(2):310-316. (IF 2.98)

15) Liu H, Shen F, Haruehanroengra P, Yao Q, Cheng Y, Chen Y, Yang C, Zhang J, Wu B, Luo Q, Cui R, Li J, Ma J, Sheng J, Gan J. A DNA Structure Containing AgI -Mediated G:G and C:C Base Pairs. Angew Chem Int Ed Engl. 2017 Aug 1;56(32):9430-9434. (IF 12.96)

16) Wu B, Li L, Huang Y*, Ma J*, Min J*. Readers, writers and erasers of N6-methylated adenosine modification. Curr Opin Struct Biol. 2017 Dec;47:67-76. (IF 6.90)

17) Liu H, Cai C, Haruehanroengra P, Yao Q, Chen Y, Yang C, Luo Q, Wu B, Li J, Ma J*, Sheng J*, Gan J*. Flexibility and stabilization of HgII-mediated C:T and T:T base pairs in DNA duplex. Nucleic Acids Res. 2017 Mar 17;45(5):2910-2918. (IF 11.5)

18) Zhang J, Liu H, Yao Q, Yu X, Chen Y, Cui R, Wu B, Zheng L, Zuo J, Huang Z*, Ma J*, Gan J*. Structural basis for single-stranded RNA recognition and cleavage by C3PO. Nucleic Acids Res. 2016 Nov 2;44(19):9494-9504. (IF 11.5)

 
更新时间:2020年09月15日

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